BT60002: Recombinant Dna Technology

Recombinant DNA Technology is an advanced level course typically taken by Dual Degree students in their final year and M.Tech and Ph.D. students in their first year coursework. The course instructors are Dr. Agneyo Ganguly, Prof. A. K. Ghosh, and Prof. A. S. Ghosh. The course has 4 lecture hours along with a 2 credit practical course.

BT60002
Course name	Recombinant Dna Technology
Offered by	Biotechnology
Credits	4
L-T-P	3-1-0
Previous Year Grade Distribution
3 5 5 5 9 6 EX A B C D P F
Semester	Spring

Syllabus

Syllabus mentioned in ERP

Tools of recombinant DNA: restriction endonucleases and other enzymes, plasmid, bacteriophage, cosmid and other vectors. c-DNA and genomic library, Gene isolation, cloning and expression, DNA sequencing, oligonucleotide synthesis, Southern and Northern hybridization, FISH, PCR, RAPD, RFLP, DNA fingerprinting and their applications for diagnosis of disease, site-directed mutagenesis, Gene silencing, Gene transfer technologies, Gene therapy; Molecular basis of genetic diseases, genetic counseling. Functional genomics: DNA chips and microarray gene screen technology; transgenic animals and gene knockout techniques, cell culture based techniques. Practical: DNA isolation and purification, restriction enzyme analysis, southern hybridization, DNA sequencing, PCR, RAPD, gene cloning, identification of recombinant clone and expression of protein in bacteria and eukaryotic cells.

Concepts taught in class

Student Opinion

• Easy to moderate level difficulty, mostly familiar concepts already covered in previous courses like Gene Expression, Immunotechnology
• No scope of novelty. Content and questions are repeated alike every year.

How to Crack the Paper

• Previous year question papers